Trans woman, patient-researcher, and the sole subject of a longitudinal N=1 case study tracking neovaginal microbiome establishment after peritoneal flap vaginoplasty. Data and protocol archived on OSF.
The cyclical hormone study is closed. A Group A Strep infection and the antibiotics that cleared it compromised the original protocol, so the project is narrowing to one question: can a peritoneal neovagina build and hold a Lactobacillus-majority microbiome?
Cycle 2
Cycle 2 Recap: The Data Is Starting to Have a Shape Cycle 2 ran April 29 through May 26, 2026 and included three serum draws, twenty-one Inito readings, and one very tired luteal phase. The short version: the protocol is producing an estradiol curve that broadly follows the shape
It's been a bit since I've posted things, but the cycle 2 data has been posted to the data site. The cycle recap and thoughts will be coming soon!
Cycle 2
I'm well into Cycle 2 at this point and overall it's going a lot better and a lot of data has been gathered, along with some various discoveries I've made about the Inito device. It was also mentioned in a separate post, but raw
Like with most things in my I'm slightly behind and should have done this sooner, but the raw data is now available at data.secondcycle.io. Ongoing data will be published after the completion of each cycle. All data will be in the same format as explained in
Cycle 1
Cycle 1 finished a week ago... I'm just now getting around to writing up the recap. Cycle 1 went okay-ish at best; the first two weeks were on protocol but things really fell apart in week three. Finding the time for multiple vaginal insertions daily during the
I'm well over two weeks into the first cycle and all in all I can't say I've noticed all that much of a difference... maybe that's how it's supposed to be or maybe my whole mind-body/somatic connection isn&
Cycle 1
Cycle 1, Day 2. Test date: April 2, 2026. The Evvy results are back, and they paint exactly the picture I expected: a microbiome that hasn't had any reason to organize itself yet. The Scores Evvy reports three summary metrics. Mine are all flagged "Out of Range&
Cycle 1
So I'm one week in, I did my first EV injection in 21 days a few days ago after my washout phase (my previous peak was at 541 pg/mL)... I definitely noticed my mood being a bit better the morning after my injection; I had an E2
Cycle 1
Today is the first day of the first cycle. My last injection was 17 days ago. The washout is done. Estradiol is sitting near trough, probably somewhere around 40-50 pg/mL based on the Inito reading this morning (E3G came in around baseline). Progesterone has been stopped. FSH is
Cycle 1
This site is live. The protocol is live. Cycle 1 has started. If you're new here, the About page explains who I am and why this project exists. The Protocol page has the full technical breakdown of the 28-day cycle, dosing, monitoring methodology, and calibration data. Start